L8902

Sigma

 

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-M, cell culture tested, lyophilized powder

Synonym:Phaseolus vulgaris agglutinin, PHA
EC Number:232-718-7

Description

Analysis NoteAgglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.
Biochem/physiol ActionsPHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.1,2
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides.3 PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin.4 PHA-M is the mucoprotein form.4 Conjugates are prepared from the corresponding purified lectins.

Properties

formlyophilized powder
potency<40 μg per mL agglutination activity
total impurities salt, free
solubilityPBS: 2 mg/mL
suitabilitycell culture tested
storage temp.2-8°C

Safety

Safety Statements22-24/25
RTECSTJ3470000

Related Products

Related productL4144, Lectin from Phaseolus vulgaris (red kidney bean)

References

Cited Reference1. Yachnin, A. and Svenson, R.H. Immunology 22, 871, (1972)
 2. Felsted, et al. J. Biol. Chem. 252, 2967, (1977)
 3. Kornfeld, R. and Kornfeld, S. J. Biol. Chem. 245, 2536, (1970)
 4. Rigas, D.A. and Osgood, E.E. J. Biol. Chem. 212, 607, (1955)